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SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…

9:06 LTE 4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.

Please include your reference below for my further research. Thank you! 1. What are the basic components of a Fluorescence Microscope and what are the functions of each?   2. Are there any parts that you can remove without compromising accuracy and utility of the equipment?  3. Can you suggest additional components to improve the equipment?

USING & FOCUSING THE MICROSCOPE: 1. List the following items into their correct order for proper set-up & use of the microscope: set scanning lens (4x), set low power lens (10x), turn on power, place slide on stage, set light to medium, look through eyepiece (= oculars), fine adjustment focus, coarse adjustment focus, center specimen 1. 2. 3. 4. 5. 6. 7. 8. 9.

A. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Power

List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…

Using a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.

PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observation

Can someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?

30 What is the magnification of the microscope shown below? Grid your answer. 10x O 000 25x

Give a particular use/uses of the following types of a microscope: 1. Research microscope 2. Stereomicroscope 3. Student microscope 4. Projection microscope

Analysis: Write your answers on the space provided. 1. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? 2. When you move the slide to the left, in what direction does the letter “e” appear to move? When you move it to the right? Up? Down? 3. How does the ink appear under the microscope compared to normal view? 4. Why does a specimen placed under the microscope have to be thin?

Non-conducting materials can be studied by scanning tunneling microscope(STM). 1.True 2. False

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 2: CARROT POTATO LAB Materials: 6 potato chunks, 6 carrots, 6 sucrose solutions (0m, 0.2m, 0.4m, 0.6m, 0.8m & 1.0 m), plastic cups, and paper towels Methodology: 1. Safety: Wear goggles while completing this lab. 2. Slice a carrot and potato into discs that are approximately 5 cm thick. 3. Use a cork borer to cut the carrots and potatoes. Do not include any skin on the cylinders. You need four apple and potato cylinders for each solution. 4. Keep a carrot and potato cylinders in a covered container until it is your turn to use the balance. 5. Determine the mass of the four cylinders together and record the mass. Put the four cylinders into the plastic cup container. 6. Label the containers with the color of the solution and your initials. 7. Pour the colored solution into the containers and cover with plastic lid. 8. Let stand overnight. 9. Remove the cores from the beakers, blot…

Instruction: FILL IN THE BLANKS.   1. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed.   2. In shifting to higher magnification, simply rotate the _________________ to the desired objective. If the image is not clear manipulate the __________________ knob.   3. The total magnification of the specimen as seen in the microscope is 400x. This means that the ocular magnification is _______ x and the ______________ is 40x.

Question:- Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.

Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.

districtIms.seattleschools.org/common-assessment-delivery/start/5362404321?action3Dd Biomolecules, Cell Theory, and Microscopes HW Q Which microscope should you use for examining a slide of an organism? Compound microscope O Stereoscopic dissecting microscope

Which of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4

Which of the follow are correct statements about microscope handling and care? 1. Always use TWO HANDS to carry the microscope.
  2. Only use LENS PAPER to clean the lenses.
Never wipe back and forth, but simply make a gentle swipe across the lens.  3. A soft cloth should be used to clean the lenses. 4. Start and finish the day’s work at the LOWEST MAGNIFICATION.   5. Start and finish the day’s work at the HIGHEST MAGNIFICATION 6. Always BEGIN examining a specimen with a LOW POWER objective.  7. Always BEGIN examining a specimen with a HIGHEST POWER objective 8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective.  9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective.  10. Unplug the microscope by pulling on the plug, not the cord.

Q1. WHAT ARE THE FUNCTION AND IMPORTANCE OF FOLLOWING PARTS OF THE MICROSCOPE?1 MIRROR2 CONDENSER3 DIAPHRAGM 4 COARSE ADJUSTMENT KNOB5 FINE ADJUSTMENT KNOB6 BODY TUBE7 OBJECTIVE LENSES8 LOW POWER OBJECTIVE9 HIGH POWER OBJECTIVE10 OIL IMMERSION 11 EYEPIECE

II.VIRTUAL MICOSCOPE 4X 4X SPECIMEN 10X 10X 40X 40X 100X 100x COARS FOCUA TME FOOR LCHT AVAN 100X MAGNIFICATIO | 4X N USED IN THE VIRTUAL MICROSCOPE OCULAR MAGNIFICATIO (10X) TOTAL MAGNIFICATIO

Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

List down 5 steps for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.

Two microscopes are available for students to use in your laboratory, with the following specifications: Table 1. Specifications of available microscopes in the laboratory. Microscope 1 Microscope 2 Eyepiece 10x 15x Objectives 4х, 10х, 40х, 100х 10х, 15х, 40х, 100х Numerical Aperture size 0.15, 0.25, 0.65, 1.4 0.25, 0.45, 0.65, 1.25 (per objective) A. Compute for the total magnification of an image under the LPO for both microscopes. B. Calculate the A and r for both microscopes given in Table 1. Find the actual size of the specimen or provide the appropriate conversions of the given sizes as needed to determine which of the two microscopes can you use for the following: 1. an image with 18x actual magnification and an apparent size of 1.04mm 2. unknown specimen that is 0.435Å 3. microorganism that is 2.1nm in size Table 2. Summary of computed values for two microscopes available in the laboratory. Microscope 1 Microscope 2 Total Magnification (LPO only) Wavelength of light (A) Limit…

* Question Completion Status: QUESTION 67 Specimens viewed through the microscope appear: O reversed (upside down) and inverted (backwards), O smaller and inverted (upside down). O inverted (bpside down). smaller. O reversed (backwards). QUESTION 68 What are the sample holders used in the spectrophotometer called? O Cuvettes O Volumetric flasks Syringes O Pipettes O Graduated cylinders

Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…

(ChooseI | Choose Where the slide sits on Holds the slide in place Used for making small adjustments to focus Condenses light before it goes through the sample The whole microscope sits upon this Used for moving around the stage and slide Used for making large adjustments to focus Does most of the magnification. These are close to the slide Provides light Magnifies the image, It is what you put your cycs near (Choose Choose

TRUE or FALSE only! 1. Microscopes are necessary for biological investigations because it allows us to study the detailed structures of an organism. 2. Microscopes must be handled with care and should be carried with both hands, one under the base and one at the objectives. 3. If you are tasked to mount a specimen of a head louse and view its overall morphology the best lens to use is the oil immersion objective lenses. 4. Objective lenses initially magnify the object’s image, form the real image and concentrate and send light rays to the condenser.

Using the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use. Record your observations and notes as vou will require this information when writing yourlab report