Direction: Read and analyze the following laboratory experiment and answer thefollowing question.PART 3: PLASMOLYSISMaterials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps,compound microscope, iodine, small knife, water, salt (5% and 10% solution)Methodology:1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife.2. Use an eye dropper to place a drop of water in the center of a microscope slide.Use the tweezers to peel a thin layer of skin tissue from the thick part of the onionwedge and place it in the center of the microscope slide.3.4.5.Add a drop of water and a drop of iodine over the onion tissue on the slide.Carefully lower a cover glass slip at an angle on the stained tissue to allow airbubbles to escape.6.Examine the prepared slide under the compound microscope at 100Xmagnification.7. Record what the cells look like.8.Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per100 ml of distilled water in a beaker. Stir until dissolved. Also prepare a 10%solution by adding 10 grams of salt per 100 ml of distilled water in another beaker.9. Use a dropper to add a few drops of the 5% solution to one side of the cover slipof your prepared slide. The 5% solution should mix with the fluid already on youronion tissue slide.10. Carefully slide the triangle shaped end of a paper towel under the opposite end ofthe cover slip. This will cause the liquid to mix more with the fluid on the slide.11. Use additional drops of 5% solution as needed to complete the introduction of thenew solution.12. Repeat steps 7 and 8.13. Use a dropper to add a few drops of the 10% solution to one side of the cover slipof your slide with the 5% solution. The 10% solution should mix with the fluidalready on your onion tissue slide.14. Carefully slide the triangle shaped end of a paper towel under the opposite end ofthe cover slip. Use additional drops of 10% solution as needed to complete theintroduction of the new solution.15. Repeat steps 7 and 8.Questions:1. What is the independent variable in the experiment? Why?2. What is the dependent variable in the experiment? Why?3. What possible variables should you be sure to hold Constant? Give three and explainwhy.4. What is your control variable? Why is this a valid control?

Note: Please always mention the subparts needed in case of multiple questions. Thank you! Osmosis is…

1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?

1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter12: Parenteral Equipment And Supplies

Section: Chapter Questions

Problem 23RQ

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PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observation
Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.
Please include your reference below for my further research. Thank you! 1. What are the basic components of a Fluorescence Microscope and what are the functions of each? 2. Are there any parts that you can remove without compromising accuracy and utility of the equipment? 3. Can you suggest additional components to improve the equipment?
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please help me make this procedure a flow chart III. Procedure: A. MicroscopyOperating Procedure:Place the microscope close to the edge of the table. Select a suitable stool so that when looking into the eyepiece, your back is straight and your neck is bent at the nape.1. Lower the body tube by turning the course focus knob until the 10x or 16mm objective reaches the downward stop.2. Look through the eyepiece and adjust the mirror to the position which provides the brightest and the most evenly illuminated field of vision which is the circular area seen in the eyepiece. Raise the condenser until its top lens at the same level as the stage. Place the slide on the stage and fasten it using the stage clips.3. Position the specimen area of the slide over the center of the stage aperture.4. Looking through the eyepiece, raise the coarse focus knob until the image appears. Focus as sharply as possible. Low power objective has a much greater depth of focus and is generally used for the…
Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…
Direction: Read and analyze the following laboratory experiment and answer the following question. PART 2: CARROT POTATO LAB Materials: 6 potato chunks, 6 carrots, 6 sucrose solutions (0m, 0.2m, 0.4m, 0.6m, 0.8m & 1.0 m), plastic cups, and paper towels Methodology: 1. Safety: Wear goggles while completing this lab. 2. Slice a carrot and potato into discs that are approximately 5 cm thick. 3. Use a cork borer to cut the carrots and potatoes. Do not include any skin on the cylinders. You need four apple and potato cylinders for each solution. 4. Keep a carrot and potato cylinders in a covered container until it is your turn to use the balance. 5. Determine the mass of the four cylinders together and record the mass. Put the four cylinders into the plastic cup container. 6. Label the containers with the color of the solution and your initials. 7. Pour the colored solution into the containers and cover with plastic lid. 8. Let stand overnight. 9. Remove the cores from the beakers, blot…
Subject : Environmental Microbiology Can u use the information given below to answer these 2 question 1.Provide an aim for this lab 2. Provide objectives DISCUSSION QUESTIONS What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope? What determines the resolving power of the lens system? What is the limit of resolution obtainable with the light microscope? How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria? What advantages does electron microscopy have over light microscopy? What are disadvantages of electron microscopy over light microscopy? #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choic
SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…
Using a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.