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- An injection contains 5 million international units (MIU) of interferon alpha-n3 (ALFERON N) proteins per milliliter. The recommended dose is 0.05 mL. The literature states that the activity of interferon alpha-n3 is approximately equal to 2.6 × 108international units/mg of protein. Calculate (a) the number of international units and (b) the micrograms of interferon alfa-n3 proteins administered per dose.1. knowing that the Control is 6.22 x 10-20 moles.channel-2sec-1 and CFTR-CRISPR: 3.84x 10-20 moles.channel-2.sec how did they get the answer and explain how? Immunostaining using Quantum Dot fluorescence reveals that there are 5 CFTR channels/μm2 in the “CONTROL” and 81 CFTR channels/μm2 in the “CFTR-CRISPR”. Calculate the number of moles of Cl-transported per channel per second for both treatments. Note that there are 1x108 μm2 in each cm2GenAlex Medical, a leading manufacturer of medical laboratory equipment, is designing a new automated system that can detect normal levels of dissolved immunoglobin A (70. to 312. mg/dL), using a blood sample that is as small as 250 µL. Calculate the minimum mass in milligrams of immunoglobin A that the new system must be able to detect. Be sure your answer has the correct number of significant digits. mg 0 x10 X
- OO HUAWEI Nova 2 Plus DUAL CAMERA An enzyme is known to transport lactose into the cell. Answer: Mutations in CFTR gene can cause O a. Cystic Fibrosis O b. HIV C. Tuberculosis O d. Malaria e. Sickle cell anemia Recombinant monoclonal antibodies are usually used for the following applications except O a. For therapeutic purposes O b. Immuofluorescence of O C. Immunohistochemistry Od. Hybridomas Oe. Western blotA 155-pound patient (height 5'9") is ordered interferon Alfa-2b IM 2 million 3 times a week. The drug is available 10 MU in 1 mL. How many mL will the patient need per week?1. A hospital pharmacy has available 2-mL prefilled syringes containing 80 mg of tobramycinand 1.5-mL prefilled syringes containing 60 mg of tobramycin. The syringes are calibratedin 0.25-mL units. Explain how you would prepare a medication order calling for 110 mgof tobramycin to be added to 100 mL of D5W for intravenous infusion. 2. A physician prescribes a 5-mcg/kg/min IV drip of dopamine for a 175-lb. patient, and thepharmacist adds an ampul of dopamine (200 mg/5 mL) to a 250-mL bottle of D5W. Whatdrip rate should be run, in drops per minute, using a minidrip set that delivers 60 drops/mL? 3. A physician orders an intravenous solution to contain 10,000 units of heparin in 1 literof 5% w/v dextrose solution to be infused at such a rate that the patient will receive500units per hour. If the intravenous set delivers 10 drops/mL, how many drops per minuteshould be infused to deliver the desired dose?
- 1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-BYou have the following reagents available Rat anti-Keratin 8 antibody specific for mouse Keratin 8, Rabbit polyclonal antibody against mouse keratin 5 and antifade reagent with DAPI to stain DNA. Both primary antibodies work best at 1:500 dilution. A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.Imagine you are designing a new candidate molecule that can be used as an anti-viral drug to treat SARS-CoV-2. List the basic steps you need to take from the idea to the market.
- You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamine34. Which of the following cells (below) is known to produce antiphagocytic M protein? 88 A. B. C. D. ●●●● E.The growth of hybridoma cells, producing a monoclonal antibody, was monitored for 4 and a half days, and the results obtained are listed in the following table: time (days) nº cel/mL x 106 ln nº cel 0 0,45 -0,79851 0,2 0,52 -0,65393 0,5 0,65 -0,43078 1 0,81 -0,21072 1,5 1,22 0,198851 2 1,77 0,57098 2,5 2,13 0,756122 3 3,55 1,266948 3,5 4,02 1,391282 4 3,77 1,327075 4,5 2,2 0,788457 What is the maximum specific rate (µmax) of growth of these cells per day? what is the doubling time of these cells (in days)?