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2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component K Conjugate Base Salt KYA Benzoic acid, CH,COOH 6.25 x 10 NaC H₂COO MUH Boric acid, H₂BO 5.81 x 10 NaH₂BO TWO Hydrogen cyanide, HCN 6.20 X 100 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00.

2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component K Conjugate Base Salt KYA Benzoic acid, CH,COOH 6.25 x 10 NaC H₂COO MUH Boric acid, H₂BO 5.81 x 10 NaH₂BO TWO Hydrogen cyanide, HCN 6.20 X 100 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00.

Chemistry: The Molecular Science
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 108QRT
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2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt KYA Benzoic acid, C6H5COOH 6.25 x 105 NaCH₂COO MUH Boric acid, H3BO3 5.81 x 107 NaH₂BO TWO Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00. After preparing the buffer, Joey decided to determine the buffer capacity of the solution by titration using 0.500 M NaOH as titrant. Given the data he obtained below, calculate the prepared buffer system's buffer capacity (B). Parameter Value/Measurement 75.0 Volume of buffer, mL Initial pH reading 7.10 Final pH reading 8.10 Final buret reading, mL 45.50 Initial buret reading, mL 33.80 A. ApH- B. AVNAOH (ML)- C. Moles of titrant- D. Buffer Capacity (B) -
Transcribed Image Text:2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt KYA Benzoic acid, C6H5COOH 6.25 x 105 NaCH₂COO MUH Boric acid, H3BO3 5.81 x 107 NaH₂BO TWO Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00. After preparing the buffer, Joey decided to determine the buffer capacity of the solution by titration using 0.500 M NaOH as titrant. Given the data he obtained below, calculate the prepared buffer system's buffer capacity (B). Parameter Value/Measurement 75.0 Volume of buffer, mL Initial pH reading 7.10 Final pH reading 8.10 Final buret reading, mL 45.50 Initial buret reading, mL 33.80 A. ApH- B. AVNAOH (ML)- C. Moles of titrant- D. Buffer Capacity (B) -
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