3) Define gel electrophoresis, including its theory and application. Describe the steps ofrunning gel electrophoresis using the following image. More detailed reading:https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresisPOWER SUPPLYCATHODEELECTROPHORETICBUFFERANODEWELLSAMPLEAGAROSEGELPOWER SUPPLYCATHODEANOCEHIGH MOLECULARWEIGHT SPECIESLOW MOLECULARWEIGHT ANALYTES

Introduction:- Electrophoresis is a biophysical technique mainly used to separate proteins and…

Answered: 3) Define gel electrophoresis,…

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter41: Radiation Therapy

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Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
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