"Biodegradation of Diesel Oil Using Bacillus Isolates" Environmental pollution is now a worldwide burning issue. There are many places in the Philippines, which are now contaminated by petrochemical sources, such as petroleum refineries and storage sites, paint manufacturing plants, gasoline service stations, gas emissions, etc. (EMB-DENR, 1997). Although the basic solution to the pollution problem is the elimination of the sources of these pollutants, there are however overriding economic, social and political factors that must be considered. An attractive remedial measure is the use of pesticide-degrading microorganisms for the environmental clean-up. Bacteria of the genus Bacillus are a group of versatile microbes known to degrade complex organic matters refractive to most microorganisms. Several species produce enzymes that catalyze the breakdown of petroleum products in water as well as in oil. These bacteria may provide the means of eliminating unwanted pollutants in the environment. This study was conducted at the National Crop Protection Center, UP Los Baños from April 1, 1997 to September 26, 1997 to: (1) isolate Bacillus species with petrochemical degrading activity from contaminated soil; (2) select Bacillus isolates with efficient degrading activity; and (3) determine Their efficacy in the biodegradation of petroleum.

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Chapter21: Phytochemicals
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"Biodegradation of Diesel Oil Using Bacillus Isolates"
Environmental pollution is now a worldwide burning issue. There are many places in the Philippines,
which are now contaminated by petrochemical sources, such as petroleum refineries and storage sites,
paint manufacturing plants, gasoline service stations, gas emissions, etc. (EMB-DENR, 1997). Although
the basic solution to the pollution problem is the elimination of the sources of these pollutants, there
are however overriding economic, social and political factors that must be considered. An attractive
remedial measure is the use of pesticide-degrading microorganisms for the environmental clean-up.
Bacteria of the genus Bacillus are a group of versatile microbes known to degrade complex organic
matters refractive to most microorganisms. Several species produce enzymes that catalyze the
breakdown of petroleum products in water as well as in oil. These bacteria may provide the means of
eliminating unwanted pollutants in the environment.
This study was conducted at the National Crop Protection Center, UP Los Baños from April 1, 1997 to
September 26, 1997 to: (1) isolate Bacillus species with petrochemical degrading activity from
contaminated soil; (2) select Bacillus isolates with efficient degrading activity; and (3) determine Their
efficacy in the biodegradation of petroleum.
Identify the following and write them on separate sheet/s of paper.
Background of the Study:
Statement of the Problem:
Significance of the Study:
Scope and Limitation
Transcribed Image Text:"Biodegradation of Diesel Oil Using Bacillus Isolates" Environmental pollution is now a worldwide burning issue. There are many places in the Philippines, which are now contaminated by petrochemical sources, such as petroleum refineries and storage sites, paint manufacturing plants, gasoline service stations, gas emissions, etc. (EMB-DENR, 1997). Although the basic solution to the pollution problem is the elimination of the sources of these pollutants, there are however overriding economic, social and political factors that must be considered. An attractive remedial measure is the use of pesticide-degrading microorganisms for the environmental clean-up. Bacteria of the genus Bacillus are a group of versatile microbes known to degrade complex organic matters refractive to most microorganisms. Several species produce enzymes that catalyze the breakdown of petroleum products in water as well as in oil. These bacteria may provide the means of eliminating unwanted pollutants in the environment. This study was conducted at the National Crop Protection Center, UP Los Baños from April 1, 1997 to September 26, 1997 to: (1) isolate Bacillus species with petrochemical degrading activity from contaminated soil; (2) select Bacillus isolates with efficient degrading activity; and (3) determine Their efficacy in the biodegradation of petroleum. Identify the following and write them on separate sheet/s of paper. Background of the Study: Statement of the Problem: Significance of the Study: Scope and Limitation
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