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- A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?Microbial secondary metabolites are compounds produced by strains of certain microbial(antimicrobial agent).such as penicillin and cephalosporin. Describe the industrial fermentation process of each of these antibiotics. 1.name of the strain used as inoculum 2.type of fermentation process (batch, fed-batch, continuous) and bioreactor used. 3.media preparation (carbon source, nitrogen source, salts, oxygen requirements) 4.Temperature and pH maintained.
- (1) Which of the following pieces of information need to be cited in a microbiology lab report?1. Pseudomonas aeruginosa is a Gram-negative bacillus Yes / No2. It is well established that P. aeruginosa is positive for both the catalase and cytochrome oxidasetests Yes / No3. A recent study found that hyperbaric oxygen treatment might increase P. aeruginosasusceptibility to antibiotics in cystic fibrosis patients Yes / No4. P. aeruginosa is an opportunistic pathogen Yes / No5. P. aeruginosa is an opportunistic pathogen that causes about 51,000 infections in the USannually Yes / NoWhat appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobI. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer. Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophile
- From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- Choose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antisepticsCreate a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?