Please give me a brief discussion on the observation (i.what can you observe, ii. what are the source(s) of contamination in this experiment? iii. how to prevent this contamination from happening?)
Please give me a brief discussion on the observation (i.what can you observe, ii. what are the source(s) of contamination in this experiment? iii. how to prevent this contamination from happening?)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter33: The Plant Body
Section: Chapter Questions
Problem 14TYK
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Question
EXPERIMENT 4: INDUCTION OF CALLUS SOMATIC EMBRYOGENESIS OF HAPLOID PLANTS
Objective:
- To prepare anther as a source of explant
- To induce callus somatic embryogenesis of haploid plants
Procedure:
- Cut off the buds and sort them into 3 developmental stages based on length of buds
- Surface sterilize the buds in 70% ethanol for 2-3 minutes, making sure the entire bud is immersed in the alcohol.
- Drain off excess alcohol from the buds and aseptically excise the anthers from each bud.
- Remove the filaments and culture the anther on one of the agar media provided. Label the stage of development of the bud on each petri dish as the anthers are cultured. Seal the Petri dishes with parafilm.
- Incubate in the dark at 26 to 28ºC for 4 to 8 weeks or until small plants can be seen growing out of the anthers. Then transfer to diffuse light.
- Record the results of the experiment in table form and submit the report.
Observation:
The culture was contaminated after 8 weeks of incubation
QUESTION: Please give me a brief discussion on the observation (i.what can you observe, ii. what are the source(s) of contamination in this experiment? iii. how to prevent this contamination from happening?)
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