Q4) What are the 3 main components required for cloning? Q5) What is 16S rDNA, and why would cloning it be useful? Q6) How big is the 16S rDNA ‘amplicon’ we would be cloning?
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Q4) What are the 3 main components required for cloning?
Q5) What is 16S rDNA, and why would cloning it be useful?
Q6) How big is the 16S rDNA ‘amplicon’ we would be cloning?
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- Q7) What are things that are common in cloning vectors? Why are they useful?Q8) Using the pCR 2.1 vector, what antibiotics would a cell with the vector be resistant to?Q9) What does B-galactosidase do?What would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?#3) Ligase catalyzes a reaction between the 5' phosphate and the 3' hydroxyl groups at the end of DNA molecules. The enzyme calf intestinal phosphatase catalyzes the removal of the 5' phosphate from DNA molecules. What would be the consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase?
- a）What two restriction sites are you going to use to clone your PCR product into the pL4440 plasmid? What are their DNA sequence? b) State the primer sequence you will use to amplify the F27C1.7 gene ready to be cloned into the pL4440 plasmid? c) How would you go about cloning this amplified DNA into pL4440? Using your knowledge of cloning list 5 important aspects of the method.Figure 2 illustrates the important elements of a cosmid.a) Briefly describe the importance of origin of replication in a cosmid and where are thecos sites derived from.b) The cloning capacity of a cosmid is up to 44 kilo base pairs. State TWO classes of DNAcloning vectors that have higher cloning capacity.Genome annotation refers to ... 1.) lining up overlapping regions in short shotgun sequencing reads to assemble larger contiguous DNA sequences (contigs/scaffolds). 2.) using long-read sequencing platforms (such as PacBio or Oxford Nanopore) to gather information about the epigenetic status of each region of a sequenced genome. 3.) the process of predicting which parts of a genome sequence code for functional products (such as protein-coding genes), what those products do, and assigning them names. 4.) sequencing messengeRNA measure the relative expression levels of genes in one or more tissue samples.
- List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?
- What Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a plasmid, and transform it into E. coli, will I be able use the transformed E. coli to make the corresponding protein? Explain why, or why not? If you decide to do this, what would your cloning strategy be?What is the purpose of molecular cloning?