QUESTION 9 What are the various ways of dispensing heparin in a biomaterial? Compare and contrast.
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- Question 7. What is the rate-limiting step in the formation of an actin microfilament?2.3 What is the meaning of "in vitro"?Question 1 Amyloid Precursor Protein (APP) is actually thought to be a natural neuroprotective agent induced by neuronal stress or injury. O True O False Question 2 Which of the following is not a property of serine protease? A globular protein with high molecular weight ● It is initially synthesized as a zymogen It has uniquely activated tyrosine residue at the active site Cleaves polypeptide at a specific amino acid residue Question 3 RTK is activated by cyclic AMP. O True False
- The allosteric regulation of ATCase by CTP is an example of: 1. Negative homotropic allostery 2. Positive homotropic allostery 3. Negative heterotropic allostery 4. Positive heterotropic allosteryQuestion 28 : In a SDS-PAGE analysis (Indicate the right answer) : A- The proteins are denatured and positively charged by the addition of SDS. B- Large proteins run faster than small proteins. C- If the pHi of a protein corresponds to the pH of the running buffer the protein becomes positively charged (+) and cannot migrate anymore into the gel. D- For reducing disulfide bridges one needs to add a reducing agent such as 2-mercaptoethanol or DTT. E- All is wrong.Question 31 In E. coli, which of the following factors function to ensure the proper binding of tRNA Met during mRNA binding and ribosomal subunit assembly? I. IF-1 II. IF-2 III. IF-3 IV. EF-Ts A) I only B) I and III C) II and IV D) I and II
- Question 4 Which of the following represents an example of a proteoglycan? attachment of several molecules of heparin to the peptide antithrombin II attachment of a Gal-B(1,3)-GalNAc disaccharide to the fish antifreeze protein attachment of a small oligosaccharide to an immunoglobulin O attachment of a small antigenic determinant to a membrane bound protein of a cellQUESTION 2 Which peptide will most strongly attach to a carboxymethyl ion exchange column at pH 7.4? O N'-Glu-Lys-Arg-Asp-Tyr-C' O N'-Tyr-Arg-Lys-Arg-Gly-C' O N'-Gly-Glu-Gln-Asp-Asp-C' O N'-Val-Ala-Leu-lle-Arg-C' QUESTION 3 Which will be product of transcription from this double-stranded (ds) DNA sequence? 5' - atgttcaaagcctattcg - 3' 3' - tacaagtttcggataagc - 5' O YKFRIS O uacaaguuucggauaagc O auauucaaaaccuauuca Which will be product of transcription from this double-stranded (ds) DNA sequence? 5' - atgttcaaagcctattcg – 3' 3' - tacaagtttcggataagc – 5' O YKFRIS O uacaaguuucggauaagc O auguucaaagccuauucg Ο MFKAYS QUESTION 4 The structure of hemoglobin consists of a 2a and 2B heterotetramer. O quaternary O tertiary O secondary O primaryQUESTION 4 What percent are the following two 20 amino acid peptides similar? MTEKRVQDKAKLIVDEQACT MTDVRIQERTKLKVFEQLCI O 25% O 25% O 75% 90%
- Question 8 Which statement about N-linked glycosylation is correct? O N-linked oligosaccharides are attached to proteins one sugar at the time in the endoplasmicreticulum. O N-linked sugars are attached to proteins as a 14 residue oligosaccharide during translation in the endoplasmic reticulum. O N-linked sugars are attached to proteins as a 14 residue oligosaccharide during translation inthe cytoplasm. O N-linked sugars are attached to proteins as a 14 residue oligosaccharide after translation inthe Golgi apparatus.(a) Draw the structure of the high-energy nucleoside triphosphate GTP. (b) Draw the structure of the hydrolysis product formed when one phosphate is removed.Question 10. What molecule in the cell is the first to recognize the signal peptide as it is translated by the ribosome?