Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c
Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c
Chapter4: Least-squares And Calibration Methods
Section: Chapter Questions
Problem 1P
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Question
Sample (μg) |
Absorbances (520 nm)
|
Concentration
|
0.0 |
0.621 |
0 |
2.5 |
0.599 |
2.5 |
5.0 |
0.556 |
5 |
10.0 |
0.469 |
10 |
15.0 |
0.418 |
15 |
20.0 |
0.291 |
20 |
- Plot a graph in which y axis represents for absorbance and x-axis represents for concentration
- From graph, y=mx + c
- Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.
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