Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
Publisher:Crouch
Chapter4: Least-squares And Calibration Methods
Section: Chapter Questions
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Sample (μg)

Absorbances (520 nm)

 

Concentration

 

0.0

0.621

0

2.5

0.599

2.5

5.0

0.556

5

10.0

0.469

10

15.0

0.418

15

20.0

0.291

20

 
  • Plot a graph in which y axis represents for absorbance and x-axis represents for concentration
  • From graph, y=mx + c
  • Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.
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