Tests Indication/Purpose Procedures/ Specific test sample used Draize Acute Skin Irritation Test Draize Eye Irritation Test Coagulation and Liquefaction of Proteins Chorioallontoic Membrane (CAM) Assay
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- Match the methods discussed to the description of what it would be used for. HPLC-UV Visual range standard curve Affinity chomatography UV analysis of protein fractions Gel filtration Ion Exchange SDSPAGE…Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstainingmead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the…
- Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition. After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.Eosin Y Test solution procedure in preparationPlease state the step-by-step procedure of SMEAR PREPARATION? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
- Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.Cause and Effect Analysis describe the effect on the test procedure and explain briefly The zones of adjacent antibiotic disks overlap. Antibiotic disk cartridge was left open for more than 7 days. Antibiotic disk was placed closely at the edge of the plate.I. EVACUATED TUBES, ANTICOAGULANTS, AND LABORATORY USES Complete the following table Additive/Anticoagulant Sodium Citrate Laboratory Test(s) Function Нерarin K2EDTA Gel Separator
- 6 2 4 3 5 LABORATORY REPORT FORM EXERCISE 25 ASSAY OF ANTIMICROBIAL AGENTS: DISK-DIFFUSION METHODS What is the purpose of this exercise? Evaluate 1. For each of the disinfectants or antiseptics tested, determine the active ingredient. What class of inhibitory agent does it belong to and what is its mode of action? CHEMICAL AGENT Lysol Sanitizer Foaming Handwash Detergent. Desinfecting Hand Sanitizer ACTIVE INGREDIENT(S) Alhyl Dinethylaenzy/a. chloroxylenol 0.3% Alkyl, Dimethyl benzyl ammonium chlorides ethy benzyl A. C. Ethyl Alcohol 70% 1/Cleansing s Pray Hydrogen Peroxide Benzalkonium Lidocaine HC) An Septic CLASS OF AGENT MODE OF ACTION1) Determine the MICS on the simulated microbroth-dilution results from an MIC Assay on Staphylococcus aureus. Legends: PC: Positive Control (No antimicrobial agent) NC: Negative Control (No bacteria) No Growth With Growth µg/mL: 128 64 32 16 8 4 2 1 0.5 0.25 PC NC 2 3 4 5 7 8 9 10 11 12 Amoxicillin A Ticarcillin Piperacillin C D Cefotaxime E Ertapenem F Nalidixic Acid G Ciprofloxacin H Gentamicin а. Fill out the MICS on the table below. MIC Susceptibility to Staphylococcus aureus Antimicrobial Agent (Hg/mL) (Susceptible, Intermediate, or Resistant) Amoxicillin Ticarcillin Piperacillin Cefotaxime Ertapenem Nalidixic Acid Ciprofloxacin Gentamicin BStep-by-step smear preparation of Kinyoun Method