The Y150F (Tyr 150 to Phe) mutant of B-lactamase catalyzes the reaction with a keat of 3.1 * 10³ s¹ at pH = 7.0 and 37 °C. Assuming this decrease in keat is due to the loss of transition state stabilization by the Y150F mutant, calculat the AAG* between the wild-type and Y150F enzymes. Show your work and state any assumptions you make in solving this problem.

The Y150F (Tyr 150 to Phe) mutant of B-lactamase catalyzes the reaction with a keat of 3.1 * 10³ s¹ at pH = 7.0 and 37 °C. Assuming this decrease in keat is due to the loss of transition state stabilization by the Y150F mutant, calculat the AAG* between the wild-type and Y150F enzymes. Show your work and state any assumptions you make in solving this problem.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter26: Synthesis And Degradation Of Nucleotides

Section: Chapter Questions

Problem 10P

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