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- Match each enzyme name in the left column with the correct descriptive phrase in the right column. a. Topoisomerase II b. DNA ligase c. DNA polymerase y d. Reverse transcriptase i. Catalyzes most nucleotide incorporations in bacterial DNA replication ii. Cleaves RNA in a DNA-RNA hybrid molecule e. DNA polymerase I f. DNA polymerase II iii. Uses a tRNA primer in synthesis of retroviral DNA iv. Acts through an adenylylated DNA inter- mediate v. Catalyzes formation of a double-strand DNA break vi. Catalyzes mitochondrial DNA replicationWhich of the following enzymes are NOT used in recombinant DNA technology O A. Eco RI O B. Ligase O C. Lysozyme O D. T4 polymerase O E. Terminal transferaseWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IV
- Four enzymes are listed below, match each to the correct definition. Definition Enzyme 4. Ligase 5. Primase 6. Gyrase 7. Helicase Answer a. Unwinds DNA b. Relieves tension in DNA during unwinding c. Links sugars and phosphates together d. Builds RNA primers C В AThe enzyme responsible for the joining of Okazaki fragments is a. helicase b. primase c. DNA ligase d. DNA topoisomeraseMolecule involved in joining segmented nucleotide strands is... O a. DNA ligase O b. Ribosomes c. RNA polymerase d. DNA polymerase ge F5 F6 F7 F10 Scrol F8 F9
- How would a PCR reaction be affected if there are no primers in the reaction? a. The PCR reaction will not commence O b. The reaction will cease after a few cycles OC PCR would proceed normally O d. Non-specific PCR of random templates will occur Clear my choiceWhich of the following molecules has RNA-dependent DNA polymerase activity? A. DNA polymerase I B. DNApolymeraseIII C. RNApolymeraseD. LigaseE. TelomeraseIf you have got the following DNA template molecules, which one of them will require more energy to break down the hydrogen bonds between the antiparallel strands? O a. ATATATATCGCGTTAAATTCTA O b. GCGCGCGCGCGCGCGCGCGCG O c. AAAAAATTTTTCCCCCGGGGG O d. TACTACACTGTGGTTAATTAAA O e. GGGGGCCCCCAATTCCCCCCC tune of proteins that heln in regulating the levels of different molecules in human body, e.g
- a. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chainsSome bacteria, through natural selection, have acquired some extremely potent enzymes that destroy viral DNA, thereby preventing the bacterial cell from becoming infected with the virus. These enzymes are called: Select one: O a. DNA polymerases O b. DNA ligases c. restriction endonucleases O d. restriction ligasesThe DNA polymerase I uses its to remove the RNA primers during DNA replication in E. coli cells. Select one: a. 3' to 5' exonuclease activity O b. 3' to 5' DNA helicase activity O c. 5' to 3' exonuclease activity O d. 5' to 3' DNA polymerase activity