Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are luciferin+O2 ⇌ oxyluciferin+light ATP ⇌ AMP+PPi, ΔGo=−31.6 kJ/mol If the overall ΔGo of the coupled reaction is -8.70 kJ/mol, what is the equilibrium constant, K, of the first reaction at 27oC?

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)
10th Edition
ISBN:9781305967359
Author:STARR
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Chapter20: The Protists
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Problem 4CT: Melvin Calvin and Andrew Benson determined the steps in the light-independent reactions of...
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Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are luciferin+O2 ⇌ oxyluciferin+light ATP ⇌ AMP+PPi, ΔGo=−31.6 kJ/mol If the overall ΔGo of the coupled reaction is -8.70 kJ/mol, what is the equilibrium constant, K, of the first reaction at 27oC?
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