A marine biologist and cancer researcher worked together to isolate the green fluorescent protein (GFP) gene from a sample of jellyfish DNA. Scientists have successfully inserted this gene into a cancerous tumor in humans in order for the tumor to glow so it can be more easily removed during surgery. Once extracted from the jellyfish, how can scientists produce multiple copies of the GFP gene for medical applications? 1. Gel electrophoresis 2. Polymerase chain reaction 3. Restriction enzyme digestion 4. Transgenic technology

A marine biologist and cancer researcher worked together to isolate the green fluorescent protein (GFP) gene from a sample of jellyfish DNA. Scientists have successfully inserted this gene into a cancerous tumor in humans in order for the tumor to glow so it can be more easily removed during surgery. Once extracted from the jellyfish, how can scientists produce multiple copies of the GFP gene for medical applications? 1. Gel electrophoresis 2. Polymerase chain reaction 3. Restriction enzyme digestion 4. Transgenic technology

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 8QP

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Similar questions

Transgenic bacteria can be used to make an alanine rich (GM) plant. Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your explanation will include the role of: Restriction enzymes, plasmids, recombinant DNA, and bacteria.
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
In biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. State the three (3) important regions of the plasmid. Elaborate your answer.
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
Scientists modified the tumor-inducing (Ti) plasmid, a naturally occurring plant plasmid found in Agrobacterium tumefaciens, to create a tool used to introduce any gene of interest into plant cells. They created a binary system because a single Ti plasmid is too large to be easily manipulated. One part of the system is a disarmed plasmid, and the second part is a transformation vector. The following sentences describe the function of key DNA elements in the system. Virulence region Genes for conjugative transfer Disarmed Ti plasmid (T-region removed) ori Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer virulence Kan (bacterial selectable marker) Amp selectable marker Plant selectable marker (e.g., herbicide resistance) Constitutive promoter T-DNA left border Place the terms in the appropriate blanks to complete the sentences. Not all terms will be used. 3' transcriptional terminator MCS (inserted gene of interest) T-region Transformation…
Animals can also be genetically modified besides bacteria. As to date, transgenic cows, goats and pigs have been created in the lab. Elaborate on TWO (2) most widely used transfection methods to genetically modify an animal cell.
A has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choice
For each of the following scenarios, indicate YES (it is cloning) or NO (it is not cloning). 6. ___________ Sperm taken from a male goat is combined with a female's egg in a petri dish. The resulting embryo is implanted into the female's uterus to develop 7. ___________ A sheep embryo, composed of 16 cells, is removed from the mother's uterus and separated into individual cells. Each cell is allowed to multiply, creating 16 separate embryos, which are then implanted in different female sheep to develop to maturity. 8. ___________ A cow with many desirable traits is stimulated with hormones to produce a number of egg cells. Each of these eggs is fertilized and implanted into a surrogate mother. 9. ___________ Cell nuclei from a recently deceased dog are placed into enucleated egg cells from another female dog. These egg cells are then placed into the uterus of an additional female surrogate dog, where it grows into a puppy.
Proteins that bind to a specific DNA sequence and help control the recruitment of RNA polymerase are called ... 1.) CRISPR/Cas9 2.) restriction enzymes 3.) transcription factors 4.) topoisomerases
Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium that produces a restriction enzyme does not cut its own DNA?
Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind to the DNA strands at a low temperature. PCR also preferentially amplify short segments of DNA. Would it be important to actually run the cDNA after the PCR on a DNA gel in order to check for a PCR product of the predicted size for the insulin gene? Why or why not? How many more genes in the plasmid (besides the insulin cDNA insert) are need to determine which bacterial cells have been transformed and to determine which transformed bacterial cells have a plasmid with the cDNA insulin insert? What is needed in the plasmid with the cDNA insert besides the gene for insulin to actually cause the bacteria to express the insulin gene and produce the insulin protein?
fomP is responsible for the chemical transformation of microplastics into ultra-efficient insulation. You take an arctic seawater sample and extract the DNA. 1. First you need to locate the gene on the bacterial chromosome. What procedure(s) would you use to identify and locate the gene? Explain how it/theywork(s). 2. Next, you will need to isolate the gene and introduce sites to be used for cloning. What would you use to make many copies of this gene? What will you need? How does it work on a molecular level?