BONUS QUESTION! Protein 1 has a pl value of 7.2. Protein 2 has a pl value of 5.6. Which protein is most likely to bind to an cation-exchange (negatively charged) resin at pH 6.5? O Protein 1. O Protein 2. O Neither protein will bind to the cation-exchange resin. O Both proteins will bind to the cation-exchange resin. O Cannot tell from the information given.
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- 2. . questions below. Assume the following pKa values: N-terminal –NH3®, 7.0; all -COOH groups, 4.0; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. Calculate the overall charge (pH 7) on the following three polypeptides and answer the (A) Ser-Tyr-Ser-Met-Glu–His–Phe–Arg-Trp-Gly-Lys-Pro–Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys–Val-Tyr-Pro-Asp-Ala -Gly- Glu--Asp-Gln- Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe (B) Ser-Tyr-Ser-Met-Glu–His–Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu–Val-Tyr-Pro-Asp- Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu–Phe-Cys-Ser-Tyr-Ser-Met–Glu–His-Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu-Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala (g) Which of the three peptides would migrate the furthest away from the anode in isoelectric fo- cusing (h) Which of the peptides would migrate the closest to the cathode in isoelectric focusing?…Choice and Preparation of a Buffer System1. Choosing the proper buffer solution In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer.Buffer solutions pKa Acetate buffer 4.73Tris- (hydroxymethy) aminomethane 8.08Phosphate buffer 7.20 2. Preparation of the chosen buffer system Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).6-73 In each case, tell which side (if either) rises and why. The solvent is water. A A Osmotic membrane (a) 1% glucose (b) 0.1 M glucose (c) 1 M NaCl (d) 1 M NaCl (e) 3% NaCl (f) 1 M NaBr (B) B 5% glucose 0.5 M glucose 1 M glucose 1 M K₂SO4 3% KC1 1 M KC1
- Need both answer ASAP. 1.Draw the structure of pppUCGAP ribonucleic acid in the ionic form that would predominate at pH 7.0. 2.Draw the structure of ppTGACpp deoxyribonucleic acid in the ionic form that would predominate at pH 7.0.Question: Estimate the pl of the tripeptide ? Step 1: Determine what can be protonated/deprotonated Abundance Step 2: Estimate where the net charge of the protein would be = 0 Step 3: Use the pka's that bracket the neutral form. +H₂N COOH تیار کیا ہے CH₂ H CC H +1 H₂N H COOH CH3 CH3 0 1 2 cook CH₂ coof H pkg= 2.19 O H H₂N No charge H H соон CH3 CH3 3 4 pka= 3.86 Coo COO™ CH₂ H H H coof CH3 CH3 pka= 4.25 COO - 2 5 6 6 Coo J. CH₂ 7 pH O H H 8 H coo- CH3 CH3 COO" 9 + Н. H3N pka= 9.82 10 pka= 9.82 pka= 3.86 CH₂ H₂N 11 H H H coo CH₂ p - 3 O=C H H H 12 H pka= 4.25 O CH3 1 CH3 coo- CH3 CH3 COO 13 COO pka= 2.19 coo 14. Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pk, = 6.0) when the other has a valine (uncharged side chain). (a) Which would be better for separation: gel electrophoresis or isoelectric focusing? Why? (b) What pH would you choose for the separation?
- ok-Personal Microsoft Edge com/maildeeplink 021 pdt Download Print Save to OneDrive 1. Draw the structure of the pentapeptide Gln-Trp-His-Glu-Tyr that would predominate in aqueous solution at pH3D10.3. (Relevant pka values are: 2.2 4.3, 6.0, 9.8, and 10.1) Acp is adiusted toNeed help, please. Answer choices are provided below for drop-down questions. 1. The overall charge of this protein at pH 7 is Drop-down answer choices are [positive, negative, or neutral] 2. In the first dimension of 2-D PAGE, this protein's isoelectric focusing point is Drop-down answer choices are [between 4 and 6, between 6 and 8, or between 8 and 10] 5. A mole of the above protein weighs ____ g. For 1 mL of a 5 mM solution of the above protein, you would need to weigh out ____ Drop-down answer choices are [g, mg, mcg, ng, or pg] (number and unit, at least one non-zero integer to the left of the decimal point, don't round)Fill in the blanks 1) The interactions between biomolecules and, proteins fold, and are exerted through the_ molecules organize themselves around letronrany. in are the most critical determinant of how Effect. This is observed when, atomic surfaces causing an unfavorable change which leads to these surfaces clustering, where they interact via (interatomic) interactions. The primary interatomic interactions that contribute to stabilizing folded proteins are and In addition, rotations about single bonds energies. The Ramachandran plot determine conformations and contribute to. (which atoms?) of the indicates the energetically favored conformations for the. polypeptide. The 3-D path of this (last answer) defines the. of the protein. 2) There are two types of "regular" secondary structure elements in folded proteins, and have low energy backbone and satisfied, in the backbone atoms.. In alpha helices, the atom of the ith residue forms an H-bond with the atom of the i+ residue. In (two…
- SOLVE!!! Calculate the total volume of the diluent that you are going to use if you have prepared a 3% red cell suspension using 0.5 mL of pRBC.5- 14. Why 15. When bacteria growing at 20°C are warmed to 30°C, are they more likely to synthesize membrane lipids with (a) saturated or unsaturated fatty acids, and (b) short-chain or long-chain fatty acids? Explain. fiah (000 none 100.Part C - Exploring antiparallel 3 sheet structure The hydrogen bonding between antiparallel sheets, also visible in the pdb by selecting the antiparallel sheet view, is shown. Locate the N- and C-termini in this image. Select the antiparallel sheet view in the pdb file. How many peptide chains are shown? O 1 O 2 3 O 4 Submit Request Answer