To purify a certain protein, you were required to prepare a 0.10 M glycine buffer at pH 9.4. Unfortunately, the stockroom has run out of glycine. However, the lab aides were able to find two 0.10 M glycine solutions with pH of 9.0 and 10.0. What volumes of the solutions must you mix to obtain 200.0 mL of the required buffer?

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To purify a certain protein, you were required to prepare a 0.10 M glycine buffer at pH
9.4. Unfortunately, the stockroom has run out of glycine. However, the lab aides were
able to find two 0.10 M glycine solutions with pH of 9.0 and 10.0. What volumes of the
solutions must you mix to obtain 200.0 mL of the required buffer?

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